1. About us
ANALYSYS AND RESEARCH CENTER - "Pioneering value creation, leading in proteomics analysis and R&D solutions for biomolecules."
With a team of highly experienced experts and cutting-edge technology, we take pride in being a pioneer in the practical application of molecular biotechnology. We not only provide accurate and timely proteomics analysis services but also accompany our clients throughout the entire research and new product development process.
With extensive experience and a deep expertise of the pharmaceutical, cosmetic, food, and agricultural sectors, we are committed to providing precise, rapid, and comprehensive molecular biology analysis solutions. Our aim is to support clients in achieving breakthroughs in research, and developing innovative products.
Proud to be at the forefront of adopting the latest technology, we are committed to offering high-quality services while ensuring information security, and adhering to ethical business regulations.
- Phone: +84 32 703 9923
- Email: Biomolecules@hoanvubio.com
- Website: https://www.hoanvubio.com
- Address: Floor 3, No. 51, Tran Phu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.
Brochure
The process of protein analysis begins with the complex proteins are broken down into peptides using specific enzymes. Subsequently, these peptides are separated based on their size and charge through chromatographic or electrophoretic techniques. Each peptide is then ionized and analyzed by MS to determine its precise mass and amino acid sequence. Finally, the obtained information is compared against known protein databases to identify the original protein.
Targeted Proteomics
A typical quantitative protein analysis workflow follows these five steps: sample preparation è protein digestion è peptide separation using high-performance liquid chromatography (HPLC) è peptide ionization and fragmentation utilizing mass spectrometry (MS) è data processing.
After proteins are hydrolyzed into peptides by specific enzymes, the resulting peptide mixture is separated on an HPLC column using an appropriate mobile phase. The target peptides are introduced into MS for ionization using electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) technique. The peptide ions are then fragmented to generate ions, from which the amino acid sequence and molecular mass of the peptide can be determined. The acquired MS data is processed using specialized software to accurately quantify peptides using methods such as multiple reaction monitoring (MRM) or parallel reaction monitoring (PRM).
3. Research & Development (R&D)
3.1. What is R&D?
Research and Development (R&D) is a continuous process of innovation and improvement that empowers organizations to develop novel and distinctive products and services. In addiotion, it focuses on improving existing products to meet the dynamic and ever-changing needs of the market.
3.2. Comprehensive R&D Solutions
We offer comprehensive, specialized, and diversified R&D solutions in molecular biotechnology, with a strong emphasis on the pharmaceutical, cosmetic, food, and agricultural industries.
- Advanced Analysis: Our team of experts leverages cutting-edge analytical technologies to investigate the structure, function, and interactions of biomolecules, thereby discovering optimal solutions.
- New Product Development: We support clients throughout the entire product lifecycle, from concept to market launch, including:
- Designing and developing innovative products grounded in robust scientific principles
- Optimizing formulations and production processes
- Ensuring compliance with stringent quality and safety standards
- Enhancement of Existing Product: We assist clients enhance the efficacy and quality of existing products through:
- Conducting detailed analysis to identify product strengths and areas for improvement.
- Proposing tailored enhancement strategies
- Performing rigorous testing and evaluation
- Consulting and Technology Transfer: We deliver expert consulting services and facilitate technology transfer to help clients enhance their internal R&D capabilities
3.3. Research and Development (R&D) Process
Our R&D process encompasses the following stages:
- Objective Setting: Establishing clear and well-defined goals for the R&D project
- Research and Development: Performing both fundamental and applied research to develop innovative formulations and processes.
- Testing and Evaluation: Evaluating product efficacy through clinical trials, animal testing, or in vitro testing.
- Manufacturing and Commercialization: Facilitating technology and supporting clients in the manufacturing and market launch process.
3.4. Benefits of Partnering with Us
- Comprehensive Solutions: We provide end-to-end R&D solutions, helping clients save time and costs.
- Expert Team: Our team consists of seasoned scientists, engineers, and molecular biology specialists with extensive experience.
- State-of-the-Art Facilities: We possess a modern laboratory system that meets international standards.
- Confidentiality Assurance: We are committed to protecting all client information. We are dedicated to safeguarding all client information with the utmost discretion.
4. Services
Proteome Mapping
Proteome mapping is a technique in which the proteome is analyzed using LC-MS: a combination of chromatographic separation and mass spectrometry.
Purified protein samples are digested and run through an extended 1D or 2D LC gradient, after which the eluate is injected into the LC-MS instrument. The MS spectra are analyzed to identify the proteins of interest.
The outcome of this protein analysis depends on the number and range of proteins present. 1D LC-MS/MS analysis will identify >1000 proteins. For more complex samples (e.g., whole-cell lysates), a more sophisticated 2D LC-MS/MS protocol is employed. First, the sample is separated by strong cation exchange chromatography, followed by reversed-phase chromatography, and 2D LC-MS/MS analysis can identify >2000 proteins in a single experiment.
Proteome Mapping Request Form
For more information, please refer to the updated documentation.
Targeted quantitative mass spectrometry (MRM/SRM)
Multiple reaction monitoring (MRM) is the most widely utilized method for quantitative analysis of analytes using LC/MS/MS workflows. In this method, specific ions are filtered through the first quadrupole and directed into the collision cell, where they are referred to as precursor or parent ions. These ions are fragmented within the collision cell. Selected fragment ions, known as product or daughter ions, are subsequently passed through the second quadrupole. The process of transitioning from precursor (parent) ion to product (daughter) ion is referred to as an ion transition.
Quantitative results obtained from GC/MS and LC/MS/MS analyses can be reported either as act numerical value or as qualitative positive/negative results, depending on whether the analyte of interest meets or exceeds a predefined threshold concentration.
MRM is primarily employed to selectively identify and quantify specific analytes, including proteins, peptides, metabolites, and lipids from various biological matrices, such as plasma, serum, and other biological samples.
MRM/SRM Analysis Request Form
For more information, please refer to the updated documentation.
Research and Development (R&D)
With our expertise and advanced capabilities, we are confident in our ability to be your trusted partner in product research and development. Please feel free to contact us for professional advice and dedicated support.
Product Research and Development (Product R&D)
Through a rigorous scientific research process, we aim to discover, develop, and refine new products while continuously improving existing ones. Product R&D serves as the foundation for your business to stay ahead of industry trends and secure a competitive market position
Product R&D Request Form
For more information, please refer to the updated documentation.
Process Research and Development (Process R&D)
The objective of Process R&D is to optimize processes for maximum efficiency. In manufacturing, this involves reducing costs, improving product quality, and rapidly responding to market demands. For services-oriented businesses, Process R&D focuses on creating exceptional customer experiences and strengthening competitiveness.
Process R&D Request Form
For more information, please refer to the updated documentation.
For further information, please refer to the updated documents available in the document section.
Protein Identification and Characterization
- Protein Identification by Mass Spectrometry
- De novo Peptide Sequencing
- Protein Mass Analysis
- MRM/SRM Analysis
Quantitative Analysis
- Quantitative Analysis of Peptides and Proteins
- Isotope Analysis
Research and Development
- Novel Molecule Discovery
- Product Research and Development
Support Services
- Project Consultation
- Additional Services
General Information
- Pricing and Ordering
- Terms and Conditions
- Downloadable Resources
Frequently Asked Questions (FAQs)
5. Development Orientation
Analysis of input and output product samples:
- Detection of allergens in food, dietary supplements, pharmaceuticals, and cosmetics.
- Monitoring of pesticides using isotopic analysis for selective toxicity, persistence, and distribution by utilizing traceability.
- Food quality and safety control.
- Testing the efficacy of drug and dietary supplement ingredients (for toxicity, drug resistance).
- Identification of peptides mimicking proteins present in pathogens.
6. References
7. Applications
The applications of proteomics are incredibly diverse and abundant. The table below lists some of these applications and provides links to examples of studies that have utilized these methods.
| Proteomics Applications | Detailed description and examples |
|---|---|
| Personalized Medicine | Developing personalized treatment plans tailored to individual patients based on their genetic and epigenetic profiles, aiming to improve therapeutic efficacy and reduce adverse effects. |
| Biological marker identification | Identifying protein biomarkers for the diagnosis and prognosis of glioblastoma multiforme and evaluating patient response to therapeutic interventions, such as stem cell transplantation. |
| Pharmaceutical research and development | Discovering potential drug targets, validating the effectiveness of therapeutic protein targets, and developing drugs targeting candidate (e.g., for hepatocellular carcinoma). |
| Biological system | Systematic investigations to identify potential biomarkers and therapeutic targets by elucidating disease pathways and host-pathogen interactions; evaluating drug efficacy, toxicity, resistance, and effectiveness through systematic assessments. |
| Agricultural science | Studying plant-pathogen interactions and employing cultivation methods to enhance plant resilience under adverse environmental conditions, such as flood, and drought. |
| Food science | Detecting allergens and optimizing nutritional value to enhance food quality and safety. |
8. Content update
- Latest news: Important events, recent achievements.
- Products/Services: Introduce details about products/services.
- Typical successes: Successful projects, customer stories.
- Articles/Reports: Links to published articles and reports.
- Support documents: Download documents, user instructions.
9. Service Request Forms
Protein Identification by Mass Spectrometry
Proteome Mapping
MRM/SRM Analysis
Peptide Sequencing
Gel Electrophoresis
Research and Development