Proteome Mapping

Proteome mapping is a technique in which the proteome is analyzed using LC-MS: a combination of chromatographic separation and mass spectrometry.

Purified protein samples are digested and run through an extended 1D or 2D LC gradient, after which the eluate is injected into the LC-MS instrument. The MS spectra are analyzed to identify the proteins of interest.

The outcome of this protein analysis depends on the number and range of proteins present. 1D LC-MS/MS analysis will identify >1000 proteins. For more complex samples (e.g., whole-cell lysates), a more sophisticated 2D LC-MS/MS protocol is employed. First, the sample is separated by strong cation exchange chromatography, followed by reversed-phase chromatography, and 2D LC-MS/MS analysis can identify >2000 proteins in a single experiment.

Proteome Mapping Request Form

For more information, please refer to the updated documentation.

Targeted quantitative mass spectrometry (MRM/SRM)

Multiple reaction monitoring (MRM) is the most widely utilized method for quantitative analysis of analytes using LC/MS/MS workflows. In this method, specific ions are filtered through the first quadrupole and directed into the collision cell, where they are referred to as precursor or parent ions. These ions are fragmented within the collision cell. Selected fragment ions, known as product or daughter ions, are subsequently passed through the second quadrupole. The process of transitioning from precursor (parent) ion to product (daughter) ion is referred to as an ion transition.

Quantitative results obtained from GC/MS and LC/MS/MS analyses can be reported either as act numerical value or as qualitative positive/negative results, depending on whether the analyte of interest meets or exceeds a predefined threshold concentration.

MRM is primarily employed to selectively identify and quantify specific analytes, including proteins, peptides, metabolites, and lipids from various biological matrices, such as plasma, serum, and other biological samples.

MRM/SRM Analysis Request Form

For more information, please refer to the updated documentation.

Research and Development (R&D)

With our expertise and advanced capabilities, we are confident in our ability to be your trusted partner in product research and development. Please feel free to contact us for professional advice and dedicated support.

Product Research and Development (Product R&D)

Through a rigorous scientific research process, we aim to discover, develop, and refine new products while continuously improving existing ones. Product R&D serves as  the foundation for your business to stay ahead of industry trends and secure a competitive market position

Product R&D Request Form

For more information, please refer to the updated documentation.

Process Research and Development (Process R&D)

The objective of Process R&D is to optimize processes for maximum efficiency. In manufacturing, this involves reducing costs, improving product quality, and rapidly responding to market demands. For services-oriented businesses, Process R&D focuses on creating exceptional customer experiences and strengthening competitiveness.

Process R&D Request Form

For more information, please refer to the updated documentation.

For further information, please refer to the updated documents available in the document section.

Protein Identification and Characterization

• Protein Identification by Mass Spectrometry
• De novo Peptide Sequencing
• Protein Mass Analysis
• MRM/SRM Analysis

Quantitative Analysis

• Quantitative Analysis of Peptides and Proteins
• Isotope Analysis

Research and Development

• Novel Molecule Discovery
• Product Research and Development

Support Services     

• Project Consultation
• Additional Services

General Information

• Pricing and Ordering
• Terms and Conditions
• Downloadable Resources